Recombinant CH25H protein (His tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

Recombinant Human Cholesterol 25-Hydroxylase Fragment (aa 142-272) is a high-purity protein fragment derived from the CH25H gene, designed for structural and biochemical studies. This 131-amino acid fragment retains key solubility and stability properties, making it an ideal tool for investigating the enzyme's role in cholesterol metabolism, antiviral defense, and immune regulation.

Cholesterol 25-hydroxylase (CH25H) is an endoplasmic reticulum membrane-bound enzyme that catalyzes the conversion of cholesterol to 25-hydroxycholesterol. This oxysterol product serves as a critical regulator of cholesterol biosynthesis by repressing sterol regulatory element-binding protein (SREBP) processing. Beyond lipid homeostasis, 25-hydroxycholesterol functions as a precursor for 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic receptor GPR183/EBI2 that guides lymphoid cell migration. As an interferon-stimulated gene, CH25H exhibits broad antiviral activity against enveloped viruses including SARS-CoV-2 by activating ACAT-mediated cholesterol internalization and inhibiting viral membrane fusion. Additionally, it plays roles in testicular Leydig cell differentiation and protects macrophages from cholesterol overload-induced inflammasome activation. The selected fragment (aa 142-272) represents a stable, soluble region optimized for experimental applications.