Cecropin 2 peptide

Cecropin 2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

GWLKKIGKKIERVGQHTRDATIQTIGVAQQAANVAATLK

Gly-Trp-Leu-Lys-Lys-Ile-Gly-Lys-Lys-Ile-Glu-Arg-Val-Gly-Gln-His-Thr-Arg-Asp-Ala-Thr-Ile-Gln-Thr-Ile-Gly-Val-Ala-Gln-Gln-Ala-Ala-Asn-Val-Ala-Ala-Thr-Leu-Lys

39

96%

Synthetic

Powder

Found in multiple species. The same peptide was also found in housefly (Liang Y et al. 2006 Dev Comp Immunol 30: 249-57). It has been recombinantly expressed in different manners. Mdc can interact with LPS, permeate membranes, and subsequently bind to DNA (Lu X et al. 2012 Appl Microbiol Biotechnol DOI 10.1007/s00253-011-3793-2). Updated 1/2012. Cecropin 2 has Antibacterial activity. Cecropin 2 was found in the Medfly, Ceratitis capitata; also housefly, Musca domestica.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Rosetto M, Manetti AG, Marchini D, Dallai R, Telford JL, Baldari CT. Sequences of two cDNA clones from the medfly Ceratitis capitata encoding antibacterial peptides of the cecropin family. Gene. 1993;134(2):241-3.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.