Rat CD6 / Cluster of Differentiation 6 (His Tag) recombinant protein

Rat CD6 / Cluster of Differentiation 6 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

CD6 antigen

TP120; CD6; FLJ44171

Q812A4

Cd6

Human Cells

A DNA sequence encoding the rat CD6 (Q812A4) (Met1-Asn396) was expressed with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat CD6 comprises 390 amino acids and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the recombinant protein is approximately 64-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Immobilized rat CD6-His at 10 μg/mL (100 μL/well) can bind rat ALCAM-Fc3 (Cat:503801), The EC₅₀ of rat ALCAM-Fc3 (Cat:503801) is 19.5-45.5 ng/ml.
2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
When 8 x 104 cells/well are added to ratCD6-His coated plates (10μg/mL, 100 μL/well), approximately 39.2% will adhere after 60 minutes at 37℃.