Recombinant CD28 protein (His tag)

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Recombinant Human CD28 (TP44) Fragment (aa 19-152) - a high-purity protein fragment encompassing the complete Ig-like V-type domain of the T-cell-specific surface glycoprotein CD28. This fragment serves as an essential tool for studying T-cell activation, immune signaling pathways, and costimulatory mechanisms in immunological research.

CD28 is a critical T-cell surface receptor that plays a fundamental role in T-cell activation, proliferation, survival, and immune homeostasis maintenance. As a costimulatory receptor, it functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events and alter T-cell gene expression programs. Engagement with its ligands CD80 or CD86 enhances T-cell proliferation and cytokine production, particularly IL2, in both CD4+ and CD8+ T-cell subsets. Mechanistically, CD28 ligation induces recruitment of protein kinase C-theta and GRB2, leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. The protein is localized to the cell membrane as a single-pass type I membrane protein, positioning it ideally for cell surface interactions and signaling. This recombinant fragment specifically targets the functional Ig-like V-type domain, making it invaluable for domain-specific functional studies and ligand interaction analyses.