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Product Name
CCNG1 Polyclonal Antibody
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Description
Polyclonal antibody to CCNG1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CCNG1 antibody; CCNG antibody; cyclin-G1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human CCNG1 (NP_004051.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IF 1:20 - 1:100 -
Validations
Western blot - CCNG1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CCNG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunofluorescence - CCNG1 Polyclonal Antibody
Immunofluorescence analysis of 293T cells using CCNG1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"