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Product Name
Recombinant CCD47 protein (His tag)
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Description
Recombinant human CCDC47 (Calumin) fragment (residues 156-483) offers a stable and soluble unit of the coiled-coil domain-containing protein 47, essential for studying its role in multi-pass membrane protein insertion and endoplasmic reticulum organization. This fragment is optimized for structural and biochemical analyses, providing researchers with a reliable tool to investigate protein translocation and ER-associated processes.
CCDC47, also known as Calumin, is a key component of the multi-pass translocon (MPT) complex in the endoplasmic reticulum membrane. It facilitates the insertion of multi-pass membrane proteins into the lipid bilayer by occluding the lateral gate of the SEC61 complex after initial transmembrane segment insertion, ensuring proper protein folding and integration. Additionally, CCDC47 regulates calcium ion homeostasis within the ER and supports protein degradation via the ER-associated degradation (ERAD) pathway. Its essential role in maintaining ER organization during embryogenesis and involvement in cellular protein quality control underscore its significance in cellular physiology and disease research.
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Protein name
PAT complex subunit CCDC47
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Uniprot ID
Q96A33
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Gene Name
CCDC47
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 156-483 aa (Q96A33) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 39.72 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 43 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"