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Product Name
CBR4 Polyclonal Antibody
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Description
Polyclonal antibody to CBR4
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Tested applications
WB, IHC
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Species reactivity
Human, Rat
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Alternative names
CBR4 antibody; SDR45C1 antibody; carbonyl reductase 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-237 of human CBR4 (NP_116172.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:20 - 1:200 -
Validations
Western blot - CBR4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CBR4 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - CBR4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using CBR4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBR4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using CBR4 antibody at dilution of 1:100 (40x lens).
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Background
The heterotetramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis. Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD. The homotetramer has NADPH-dependent quinone reductase activity. Both homotetramer and the heterotetramer have broad substrate specificity and can reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"