-
Product Name
CAV2 Monoclonal Antibody
- Documents
-
Description
Monoclonal antibody to CAV2
-
Tested applications
WB, IHC, IF, IP
-
Species reactivity
Human
-
Alternative names
CAV2 antibody; CAV antibody; caveolin-2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant protein of human CAV2
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50 -
Validations
Western blot - CAV2 Monoclonal Antibody
Western blot analysis of extracts of HUVEC cells, using CAV2 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - CAV2 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human cervix using CAV2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CAV2 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human uterus using CAV2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - CAV2 Monoclonal Antibody
Immunofluorescence analysis of HeLa cells using CAV2 antibody .
-
Background
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"