Cathelicidin-RC1 peptide

Cathelicidin-RC1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

KKCKFFCKVKKKIKSIGFQIPIVSIPFK

Lys-Lys-Cys-Lys-Phe-Phe-Cys-Lys-Val-Lys-Lys-Lys-Ile-Lys-Ser-Ile-Gly-Phe-Gln-Ile-Pro-Ile-Val-Ser-Ile-Pro-Phe-Lys

28

96.5%

Synthetic

Powder

51.35% similar to AP2424 Crotalicidin. More similar to reptile cathelicidin than to other amphibian peptides. Rich in K (32%). Active against Gram- E. coli (MIC 2.8-5.7 uM), S. dysenteriae, K. peneumoniae (5.7-22 uM, >200 for one strain), K. oxytoca (1.43 uM), P. mirabilis (5.7 uM), S. maltophilia (11.4 uM), S. maltophilia, P. aeruginosa ATCC27853 (2.9 uM), Gram+ bacteria S. aureus (3 active MIC 2.9 uM; 3 inactive MIC>200 uM), B. subtilis, S. epidermidis, E. faecium (2.9 uM), N. asteroides, E. faecalis (MIC 22.8 uM), and fungi C. albicans (MIC 2.9 to >200 uM). Activity against E. coli ATCC25922 is salt-sensitive (4.7 uM no salt, 4.7 uM at 100 uM NaCl, 18.8 uM at 400 mM NaCl). Cathelicidin-RC1 was found in the American bullfrog Rana catesbeiana , North America.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Guo C, Hu Y, Li J, et al. Identification of multiple peptides with antioxidant and antimicrobial activities from skin and its secretions of Hylarana taipehensis, Amolops lifanensis, and Amolops granulosus. Biochimie. 2014;105:192-201.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.