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Product Name
CAT-1 antibody
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Description
CAT-1 Rabbit Polyclonal antibody. Positive WB detected in rat brain/liver tissue, HepG2 cells, Jurkat cells, mouse heart tissue, mouse liver tissue. Positive IHC detected in human osteosarcoma tissue. Observed molecular weight by Western-blot: 67-70kd
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATRC1 antibody; CAT 1 antibody; CAT1 antibody; HCAT1 antibody; REC1L antibody; SLC7A1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CAT-1 recombinant protein (Accession Number: XM_017020716). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
WB result of Catalog No:108883 from Dilwar in Rat tissue. Rat brain (a), heart (b) liver (c) mitochondria and liver homogenate (d), (40 ug with 10% PAGE) probed with 1:1000 dilution.
Immunohistochemical of paraffin-embedded human osteosarcoma using Catalog No:108883(SLC7A1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human osteosarcoma using Catalog No:108883(SLC7A1 antibody) at dilution of 1:100 (under 40x lens)
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:108883(SLC7A1 antibody) at dilution of 1:800
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Background
SLC7A1 represents solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
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References
- Cannon JR, Sew T, Montero L, Burton EA, Greenamyre JT. Pseudotype-dependent lentiviral transduction of astrocytes or neurons in the rat substantia nigra. Experimental neurology. 228(1):41-52. 2011.
- Calder MD, Watson PH, Watson AJ. Culture medium, gas atmosphere and MAPK inhibition affect regulation of RNA-binding protein targets during mouse preimplantation development. Reproduction (Cambridge, England). 142(5):689-98. 2011.
- Kohlhaas CF, Morrow VA, Jhakra N. Insulin rapidly stimulates L-arginine transport in human aortic endothelial cells via Akt. Biochemical and biophysical research communications. 412(4):747-51. 2011.
- Ochiai H, Moriyama J, Kanemaki N, Sato R, Onda K. Analysis of cationic amino acid transport activity in canine lens epithelial cells. Experimental animals / Japanese Association for Laboratory Animal Science. 62(4):311-7. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"