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Product Name
Mouse Carboxypeptidase A2/CPA2 (His Tag) recombinant protein
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Description
Human Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
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Protein name
Carboxypeptidase A2
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Protein short names
CPA2; CARBOXYPEPTIDASE A2
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Uniprot ID
Q504N0
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Gene Name
Cpa2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse CPA2 (Q504N0) (Met 1-Tyr 417) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse CPA2 (pro form) comprises 412 amino acids and has a calculated molecular mass of 46.6 kDa. It migrates as an approximately 47 kDa in SDS-PAGE under reducing conditions.
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Purity
> 92 % as determined by SDS-PAGE
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Activity
Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of DTNB.
The specific activity is >4000 pmoles/min/μg. -
Validations
Mouse Carboxypeptidase A2 / CPA2 Protein (His Tag) SDS-PAGE
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