-
Product Name
CAPZA2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to CAPZA2
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse
-
Alternative names
CAPZA2 antibody; CAPPA2 antibody; CAPZ antibody; F-actin-capping protein subunit alpha-2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human CAPZA2 (NP_006127.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CAPZA2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CAPZA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - CAPZA2 Polyclonal Antibody
Immunofluorescence analysis of HepG2 cells using CAPZA2 antibody . Blue: DAPI for nuclear staining.
-
Background
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"