Human CAMK2B (His & GST Tag) recombinant protein

Human CAMK2B (His & GST Tag) recombinant protein

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Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

Calcium/calmodulin-dependent protein kinase type II subunit beta

MGC29528; CAMKB; CAMK2B; CAMK2D; CAMK2; RP23-340E18.5; CAM2; MGC90738

Q13554

CAMK2B; CAM2; CAMK2; CAMKB

Baculovirus-Insect Cells

A DNA sequence encoding the human CAMK2B isoform 2 (Q13554-3) (Met 1-Gln 503) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human CAMK2B/GST chimera consists of 740 amino acids and has a calculated molecular mass of 84.2 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.

> 60 % as determined by SDS-PAGE

The specific activity was determined to be 300 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.