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Product Name
Recombinant CAH5B protein (His tag)
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Description
Recombinant Human Carbonic Anhydrase 5B (CA5B) Fragment (60-317) is a high-purity, stable protein designed for structural and biochemical studies. This 258-amino acid fragment retains key functional properties, offering researchers a reliable tool to investigate mitochondrial carbonic anhydrase activity and its role in cellular metabolism.
Carbonic Anhydrase 5B (CA5B) is a mitochondrial enzyme that catalyzes the reversible hydration of carbon dioxide to bicarbonate, a reaction critical for pH regulation, metabolic processes, and mitochondrial function. As a mitochondrial carbonic anhydrase, it plays a vital role in cellular respiration and energy production. The full-length protein is localized exclusively within the mitochondrion, underscoring its importance in organelle-specific biochemical pathways. The provided fragment (residues 60-317) represents a stable, soluble domain optimized for experimental applications, enabling detailed analysis of the enzyme's catalytic mechanisms and structural features without compromising functional relevance.
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Protein name
Carbonic anhydrase 5B, mitochondrial
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Uniprot ID
Q9Y2D0
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Gene Name
CA5B
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 60-317 aa (Q9Y2D0) were fused with 6His tag.
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Sequence
60-317
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 31.18 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 32 kDa band.
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Purity
>88%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"