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Product Name
C1R Polyclonal Antibody
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Description
Polyclonal antibody to C1R
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
C1R antibody; EDSPD1 antibody; complement C1r antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 466-705 of human C1R (NP_001724.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - C1R Polyclonal Antibody
Western blot analysis of extracts of mouse bone marrow, using C1R antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunofluorescence - C1R Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using C1R antibody . Blue: DAPI for nuclear staining.
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Background
C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"