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Product Name
Recombinant BUB3 protein (His tag)
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Description
Recombinant Human Mitotic checkpoint protein BUB3 (BUB3) fragment (residues 1-328) is a high-quality, soluble protein designed for structural and biochemical studies. This fragment represents a stable, functional unit ideal for investigating spindle-assembly checkpoint signaling and kinetochore-microtubule interactions.
Mitotic checkpoint protein BUB3, encoded by the BUB3 gene in Homo sapiens, plays a dual role in spindle-assembly checkpoint signaling and the establishment of correct kinetochore-microtubule attachments. It is essential for promoting stable end-on bipolar attachments and localizing BUB1 to kinetochores. The BUB1/BUB3 complex inhibits the anaphase-promoting complex/cyclosome (APC/C) during spindle-assembly checkpoint activation by phosphorylating CDC20, thereby regulating chromosome segregation. BUB3 is primarily located in the nucleus, specifically at the centromere and kinetochore, where it localizes from prometaphase I until the metaphase I-anaphase I transition, underscoring its critical function in cell division fidelity.
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Protein name
Mitotic checkpoint protein BUB3
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Uniprot ID
O43684
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Gene Name
BUB3
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-328 aa (O43684) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 38.81 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 39 kDa band.
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Purity
>87%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"