Recombinant BMI1 protein (His tag)

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This recombinant protein product features a stable and soluble fragment (amino acids 1-232) of the human Polycomb complex protein BMI-1, also known as Polycomb group RING finger protein 4. This fragment is optimized for structural and biochemical studies, offering researchers a reliable tool to investigate the mechanisms of epigenetic regulation and gene silencing mediated by the PRC1 complex.

The full-length BMI-1 protein, encoded by the BMI1 gene, is a critical component of the Polycomb group (PcG) multiprotein PRC1-like complex. This complex is essential for maintaining the transcriptionally repressive state of numerous genes, including Hox genes, throughout development. It functions through chromatin remodeling and histone modification, specifically mediating the monoubiquitination of histone H2A at lysine 119, which leads to heritable changes in chromatin expressibility. Within the PRC1-like complex, BMI-1 regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2. The protein is primarily localized in the nucleus, though it has also been detected in the cytoplasm, underscoring its multifaceted role in cellular processes.