BMAP-28 (BMAP28) peptide

BMAP-28 (BMAP28) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GGLRSLGRKILRAWKKYGPIIVPIIRIG

H-Gly-Gly-Leu-Arg-Ser-Leu-Gly-Arg-Lys-Ile-Leu-Arg-Ala-Trp-Lys-Lys-Tyr-Gly-Pro-Ile-Ile-Val-Pro-Ile-Ile-Arg-Ile-Gly-OH

28

96%

C₁₄₇H₂₅₂N₄₄O₃₁

3131.8

Synthetic

Powder

Active against E. coli, S. typhimurium, P. aeruginosa, S. marcescens, S. aureus (MRSA), S. epidermidis, B. megaterium (MIC 0.5-4 uM), C. albicans, and C. neoformans (MIC 4-8 uM). BMAP-28 has broad antimicrobial activities and confers protection in an animal model:mouse of bacterial infection or sepsis (Giacometti A et al. 2004 Crit Care Med 32, 2485-90). Furthermore, BMAP-28 and its isomers demonstrated anti-leishmanial activities against intracellular L. major amastigotes within a macrophage infection model. Interestingly, the D-amino acid form is more active against parasites than either BMAP-28 (the L form) or the sequence reversed form (Lynn MA et al 2011 PLoS Negl Trop Dis. 5(5):e1141). Trucation of the C-terminal tail reduced hemolysis. Of note, a modified peptide (mBMAP-28) was also synthesized by replacing the C-terminal hydrophobic tail (residues 20-28) with a more hydrophilic sequence QATPATRQ also reduced peptide cell selectivity. Updated 7/2011. BMAP-28 (BMAP28) has Antibacterial, Antifungal activity. The source of BMAP-28 (BMAP28) is Cow Bos taurus.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Skerlavaj B, Gennaro R, Bagella L, Merluzzi L, Risso A, Zanetti M. Biological characterization of two novel cathelicidin-derived peptides and identification of structural requirements for their antimicrobial and cell lytic activities. J Biol Chem. 1996;271(45):28375-81.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.