BMAP-27 peptide

BMAP-27 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GRFKRFRKKFKKLFKKLSPVIPLLHL-NH2

H-Gly-Arg-Phe-Lys-Arg-Phe-Arg-Lys-Lys-Phe-Lys-Lys-Leu-Phe-Lys-Lys-Leu-Ser-Pro-Val-Ile-Pro-Leu-Leu-His-Leu-NH2

26

95.2%

C₁₅₈H₂₆₃N₄₅O₂₇

3225.05

Synthetic

Powder

BMAP-27 is a membranolytic helical peptide. Upon interaction with bacterial membranes, BMAP-27 acquires an amphipathic α-helical conformation, with the polar sector of the cylinder characterized by the presence of numerous positively charged residues, and the opposite one having a markedly hydrophobic character. This configuration favours interaction with, and insertion into, the bacterial membrane, and the peptides then aggregate to form pores and/or disaggregates the membranes by the so-called “carpet mechanism” in a concentration dependent manner. BMAP-27 is induced to adopt a α-helical conformation in the presence of EPOLs from BF produced by all three strains, KpTs101, KpTs113, and KpMn7, but to different extents.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Bellich B, Lagatolla C, Tossi A, Benincasa M, Cescutti P, Rizzo R. Influence of Bacterial Biofilm Polysaccharide Structure on Interactions with Antimicrobial Peptides: A Study on Klebsiella pneumoniae. Int J Mol Sci. 2018;19(6)

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.