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  • BMAP-27

BMAP-27

Cat.#: 318783

Size:
Optional Service: TFA RemovalWhat's this?

Special Price 204.7 USD

Availability: 3 weeks
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Product Information

  • Product Name
    BMAP-27
  • Documents
  • Quantity/Unit
    1 Vial
  • Sequence
    H-GRFKRFRKKFKKLFKKLSPVIPLLHL-Amidation
  • Three letter code
    H-Gly-Arg-Phe-Lys-Arg-Phe-Arg-Lys-Lys-Phe-Lys-Lys-Leu-Phe-Lys-Lys-Leu-Ser-Pro-Val-Ile-Pro-Leu-Leu-His-Leu-Amidation
  • Length (aa)
    26
  • Peptide Purity (HPLC)
    95.2
  • Molecular Formula
    C158H263N45O27
  • Molecular Weight
    3225.05
  • Source
    Synthetic
  • Additional Information
    BMAP-27 is a membranolytic helical peptide. Upon interaction with bacterial membranes, BMAP-27 acquires an amphipathic α-helical conformation, with the polar sector of the cylinder characterized by the presence of numerous positively charged residues, and the opposite one having a markedly hydrophobic character. This configuration favours interaction with, and insertion into, the bacterial membrane, and the peptides then aggregate to form pores and/or disaggregates the membranes by the so-called “carpet mechanism” in a concentration dependent manner. BMAP-27 is induced to adopt a α-helical conformation in the presence of EPOLs from BF produced by all three strains, KpTs101, KpTs113, and KpMn7, but to different extents.
  • Storage Guidelines
    Ideally BMAP-27 should be stored in a freezer at or below -9C. BMAP-27 should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
  • References
    • Bellich B, Lagatolla C, Tossi A, Benincasa M, Cescutti P, Rizzo R. Influence of Bacterial Biofilm Polysaccharide Structure on Interactions with Antimicrobial Peptides: A Study on Klebsiella pneumoniae. Int J Mol Sci. 2018;19(6)
  • About TFA salt

    Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

    TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

    TFA Removal Service is recommended for:

    • Peptides that will be used in cellular assays
    • Peptides that will be used as APIs or in manufactured products
    • For hydrophilic peptides containing numerous basic residues

Peptide Property

  • Analysed Sequence:H-GRFKRFRKKFKKLFKKLSPVIPLLHL-Amidation
  • Chemical Formula:C158H263N45O27
  • Sequence length:26
  • Extinction coefficient:0 M-1cm-1
  • GRAVY:-0.37
  • Mw average:3225.05
  • Theoretical pI:12.73
  • Data Source:Peptide Property Calculator

GRAVY = grand average of hydropathy

Red: Hydrophobic uncharged residues, like F I L M V W A and P

Blue: Basic residues, like R K H and N-terminal -NH2

Green: Acidic residues, like D E and C-terminal -COOH

Black: Polar uncharged residues, like G S T C N Q and Y

Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"