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Product Name
BIN2 antibody
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Description
BIN2 Rabbit Polyclonal antibody. Positive IHC detected in human spleen tissue, human heart tissue, human lung tissue, human ovary tissue, human skin tissue. Positive WB detected in Jurkat cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 80-90 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
BIN2 antibody; BRAP 1 antibody; BRAP1 antibody; bridging integrator 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BIN2 recombinant protein (Accession Number: NM_016293). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:117147(BIN2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human spleen using Catalog No:117147(BIN2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:117147(BIN2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-BIN2 (IP:Catalog No:117147, 4ug; Detection:Catalog No:117147 1:400) with Jurkat cells lysate 2000ug.
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Background
BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)
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