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  • BIN1 antibody
  • BIN1 antibody
  • BIN1 antibody
  • BIN1 antibody

BIN1 antibody

Cat.#: 108501

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Special Price 239.0 USD

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Product Information

  • Product Name
    BIN1 antibody
  • Documents
  • Description
    BIN1 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in Jurkat cells, mouse brain tissue. Positive IHC detected in human osteosarcoma tissue. Observed molecular weight by Western-blot: 60-65 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    AMPH2 antibody; Amphiphysin II antibody; Amphiphysin like protein antibody; AMPHL antibody; BIN1 antibody; bridging integrator 1 antibody; DKFZp547F068 antibody; SH3P9 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of BIN1 recombinant protein (Accession Number: BC004101). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:117146(BIN1 antibody) at dilution of 1:500

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:117146(BIN1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human osteosarcoma using Catalog No:117146(BIN1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human osteosarcoma using Catalog No:117146(BIN1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human osteosarcoma using Catalog No:117146(BIN1 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human osteosarcoma using Catalog No:117146(BIN1 antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-BIN1 (IP:Catalog No:117146, 4ug; Detection:Catalog No:117146 1:500) with mouse brain tissue lysate 3440ug.

    IP Result of anti-BIN1 (IP:Catalog No:117146, 4ug; Detection:Catalog No:117146 1:500) with mouse brain tissue lysate 3440ug.

  • Background
    BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternate splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).
  • References
    • Giridharan SS, Cai B, Vitale N, Naslavsky N, Caplan S. Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis. Molecular biology of the cell. 24(11):1776-90, S1-15. 2013.
    • Jia Y, Wang H, Wang Y. Low expression of Bin1, along with high expression of IDO in tumor tissue and draining lymph nodes, are predictors of poor prognosis for esophageal squamous cell cancer patients. International journal of cancer. 137(5):1095-106. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"