BHB-Phe peptide

BHB-Phe peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

BHB-Phe

N-beta-hydroxybutyryl phenylalanine

1

99.48%

C₁₃H₁₇NO₄

251.3

Synthetic

Powder

β-hydroxybutyryl-phenylalanine (BHB-Phe) is an endogenous ketone metabolite of the BHB-amino acid family, produced through the "BHB shunt"—a secondary metabolic pathway of β-hydroxybutyrate (BHB). It is synthesized by the enzyme CNDP2 catalyzing the conjugation of BHB and the essential amino acid phenylalanine, with phenylalanine being the most efficient substrate for this reaction. Detectable in the plasma and tissues of mice and humans, BHB-Phe levels increase 2–10-fold under ketosis-inducing conditions and decrease in mice with impaired hepatic BHB production, reflecting its dependence on upstream ketogenesis pathways. Pharmacologically administered BHB-Phe activates specific hypothalamic and brainstem neuronal populations related to metabolic homeostasis via unique mechanisms independent of major feeding-regulatory pathways. Conserved across mammals, BHB-Phe is also synthesized by human CNDP2 and detectable in human plasma with elevated levels after exogenous ketone supplementation, confirming it as a phylogenetically conserved, ketosis-responsive metabolite involved in BHB-mediated metabolic signaling.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Moya-Garzon MD, Wang M, Li VL, et al. A β-hydroxybutyrate shunt pathway generates anti-obesity ketone metabolites. Cell. Published online November 7, 2024. doi:10.1016/j.cell.2024.10.032
• Li VL, He Y, Contrepois K, et al. An exercise-inducible metabolite that suppresses feeding and obesity. Nature. 2022;606(7915):785-790. doi:10.1038/s41586-022-04828-5

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.