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Product Name
Bcl-XL antibody
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Description
Bcl-XL Mouse Monoclonal antibody. Positive IHC detected in human testis tissue, human colon tissue. Positive IF detected in Hela cells. Positive WB detected in Jurkat cells, A431 cells, HeLa cells. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Apoptosis regulator Bcl X antibody; BCL2L1 antibody; Bcl-XL antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Bcl-XL recombinant protein (Accession Number: XM_011528964). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107081(Bcl-xL antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:107081(Bcl-xL antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:107081(Bcl-xL antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using BCL2L1 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
BCL2L1 is a member of he BCL-2 protein family. BCL2L1 is expressed as two distinct isoforms, Bcl-xL and Bcl-xS, and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.
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References
- Zhang F, Liu B, Wang Z. A novel regulatory mechanism of Pim-3 kinase stability and its involvement in pancreatic cancer progression. Molecular cancer research : MCR. 11(12):1508-20. 2013.
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