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Product Name
BCAT2 antibody
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Description
BCAT2 Rabbit Polyclonal antibody. Positive IHC detected in human skin tissue, human placenta tissue. Positive WB detected in mouse kidney tissue, HEK-293 cells, HeLa cells, HepG2 cells, human heart tissue, L02 cells, MCF7 cells, mouse 3T3-L1 cells/human fat biopsy tissue, rat heart tissue, rat kidney tissue. Observed molecular weight by Western-blot: 38-42 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BCAT2 antibody; ECA40 antibody; Placental protein 18 antibody; PP18 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BCAT2 recombinant protein (Accession Number: NM_001190). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
WB results showed 37-42kd band in mouse 3T3-L1 adipocyte lysate(Lane 1) and human fat biopsy lysate (Lane 2) from Dr. Ping Wang.
Immunohistochemical of paraffin-embedded human skin using Catalog No:108383(BCAT2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:108383(BCAT2 antibody) at dilution of 1:50 (under 40x lens)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:108383(BCAT2 antibody) at dilution of 1:800
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Background
BCAT2 represents branched chain aminotransferase 2, mitochondrial
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References
- Liu S, Miriyala S, Keaton MA. Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells. PloS one. 9(1):e85702. 2014.
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