Recombinant B3GLT protein (His tag)

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Recombinant Beta-1,3-glucosyltransferase (B3GLCT) fragment (residues 53-498) from Homo sapiens, a stable and soluble unit designed for structural and biochemical studies. This fragment retains key functional regions, enabling research into O-glucosyltransferase activity and O-fucosylglycan elongation mechanisms.

Beta-1,3-glucosyltransferase (B3GLCT) is an O-glucosyltransferase that catalyzes the transfer of glucose to fucose via a beta-1,3 linkage, specifically modifying O-linked fucosylglycans on thrombospondin type-1 (TSP) domains. This enzymatic activity is essential for the elongation of O-fucosylglycans, which play critical roles in protein modification and cellular signaling pathways. The full-length protein is localized to the endoplasmic reticulum membrane as a single-pass type II membrane protein, reflecting its involvement in the secretory pathway and glycosylation processes. The provided fragment (residues 53-498) was computationally optimized to enhance solubility and stability, facilitating experimental applications without the transmembrane region.