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  • Human CD22 (aa 176-687, His Tag) recombinant protein

Human CD22 (aa 176-687, His Tag) recombinant protein

Cat.#: 502730


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Product Information

  • Product Name
    Human CD22 (aa 176-687, His Tag) recombinant protein
  • Documents
  • Description
    CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.
  • Protein name
    B-cell receptor CD22
  • Protein short names
    MGC130020; SIGLEC2; LYB-8; FLJ22814; A530093D23; SIGLEC-2; CD22; LYB8
  • Uniprot ID
  • Gene Name
    CD22; SIGLEC2
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human CD22 (Q32M46) (Trp176-Arg687) was fused with a polyhistidine tag at the C-terminus.
  • Protein Species
  • Molecular weight
    The recombinant human CD22 consists of 523 amino acids and predictes a molecular mass of 58.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant protein is approximately 90-100 kDa due to glycosylation.
  • Purity
    > 90 % as determined by SDS-PAGE
  • Validations

    Human Siglec-2 / CD22 Protein (aa 176-687, His Tag) SDS-PAGE

    Human Siglec-2 / CD22 Protein (aa 176-687, His Tag) SDS-PAGE