AXL antibody

Cat.#: 108519

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Product Information

  • Product Name
    AXL antibody
  • Documents
  • Description
    AXL Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive WB detected in HepG2 cells, HeLa cells, HT-1080 cells, human liver tissue. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 140 kDa
  • Tested applications
    ELISA, WB, IP, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    AXL antibody; AXL oncogene antibody; AXL receptor tyrosine kinase antibody; JTK11 antibody; UFO antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of AXL recombinant protein (Accession Number: NM_021913). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:117080(AXL antibody) at dilution of 1:1500

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:117080(AXL antibody) at dilution of 1:1500

    IP Result of anti-AXL (IP:Catalog No:117080, 5ug; Detection:Catalog No:117080 1:1000) with HepG2 cells lysate 9000ug.

    IP Result of anti-AXL (IP:Catalog No:117080, 5ug; Detection:Catalog No:117080 1:1000) with HepG2 cells lysate 9000ug.

    1X10^6 HeLa cells were stained with 0.2ug AXL antibody (Catalog No:117080, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug AXL antibody (Catalog No:117080, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. This antibody detects a single band at 140 kDa, which is considered to represent the auto-phosphorylated form of endogenous Axl (PMID: 12730092).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"