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Product Name
AURKC Polyclonal Antibody
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Description
Polyclonal antibody to AURKC
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
AURKC antibody; AIE2 antibody; AIK3 antibody; ARK3 antibody; AurC antibody; HEL-S-90 antibody; SPGF5 antibody; STK13 antibody; aurora-C antibody; aurora kinase C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human AURKC
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - AURKC Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AURKC antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - AURKC Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using AURKC antibody (40x lens).
Immunohistochemistry - AURKC Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using AURKC antibody (40x lens).
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Background
Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Plays also a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. AURKC phosphorylates the CPC complex subunits BIRC5/survivin and INCENP leading to increased AURKC activity. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"