Recombinant ATPB protein (His tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

Recombinant Human ATP synthase F(1) complex subunit beta (ATP5F1B) fragment (residues 59-529) is a high-purity protein designed for structural and biochemical studies. This 471-amino acid fragment represents a stable and soluble unit of the catalytic core of mitochondrial ATP synthase, enabling detailed investigation of ATP synthesis mechanisms.

ATP synthase F(1) complex subunit beta is the catalytic component of mitochondrial ATP synthase (Complex V), which generates ATP from ADP using the proton gradient created by the respiratory chain. Located on the matrix side of the mitochondrial inner membrane as a peripheral membrane protein, it forms the catalytic core with subunit alpha. During ATP synthesis, the catalytic domain couples with proton translocation through a rotary mechanism in the central stalk. While the full-length protein naturally functions only in ATP synthesis, its hydrolytic activity can be experimentally activated in vitro. This fragment provides a valuable tool for studying the fundamental energy production processes in human mitochondria.