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Product Name
ATP7B Polyclonal Antibody
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Description
Polyclonal antibody to ATP7B
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
ATP7B antibody; PWD antibody; WC1 antibody; WD antibody; WND antibody; copper-transporting ATPase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1055-1354 of human ATP7B (NP_001230111.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ATP7B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATP7B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - ATP7B Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ATP7B antibody . Blue: DAPI for nuclear staining.
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Background
Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"