-
Product Name
ATP1B1 antibody
- Documents
-
Description
ATP1B1 Rabbit Polyclonal antibody. Positive FC detected in HEK-293 cells. Positive IF detected in HEK-293 cells. Positive IHC detected in human brain tissue, human skeletal muscle tissue. Positive WB detected in human heart tissue, human brain tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 52 kDa
-
Tested applications
ELISA, WB, IHC, FC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ATP1B antibody; ATP1B1 antibody; Beta 1 subunit of Na(+) K(+) ATPase antibody; Na+/K+ ATPase beta 1 subunit antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of ATP1B1 recombinant protein (Accession Number: NM_001677). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108344(ATP1B1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human brain using Catalog No:108344(ATP1B1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:108344(ATP1B1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-ATP1B1 (IP:Catalog No:108344, 3ug; Detection:Catalog No:108344 1:1000) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of HEK-293 cells using Catalog No:108344(ATP1B1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HEK-293 cells were stained with 0.2ug ATP1B1 antibody (Catalog No:108344, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
-
Background
ATP1B1 represents ATPase, Na+/K+ transporting, beta 1 polypeptide
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"