Recombinant ATAT protein (His tag)

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Recombinant Alpha-tubulin N-acetyltransferase 1 (Alpha-TAT1) fragment (residues 1-192) from Homo sapiens, encompassing the complete N-acetyltransferase domain. This high-purity protein is an essential tool for investigating tubulin acetylation mechanisms, microtubule dynamics, and cellular processes such as cell motility and ciliary assembly.

Alpha-Tubulin N-acetyltransferase 1 (Alpha-TAT1), encoded by the ATAT1 gene, specifically acetylates Lys-40 in alpha-tubulin within the microtubule lumen. This enzyme promotes microtubule destabilization and accelerates microtubule dynamics, with its acetylation activity being notably slow due to a non-optimized catalytic site. Alpha-TAT1 enters microtubules through their ends and diffuses rapidly throughout the lumen, preferentially acetylating long-lived microtubules. It is critical for sperm flagellar function, directional cell locomotion, chemotaxis via AP2A2-dependent acetylation at clathrin-coated pits, and may facilitate primary cilium assembly. Subcellularly, it localizes to the cytoplasm, cytoskeleton (including spindles), clathrin-coated pits, focal adhesions, and axons, highlighting its diverse roles in cellular structure and movement.