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Product Name
Recombinant ASAH1 protein (His tag)
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Description
Recombinant Acid Ceramidase (EC 3.5.1.23) Fragment from Caenorhabditis elegans, encompassing residues 17-393. This fragment is engineered for stability and solubility, making it ideal for structural studies and biochemical assays involving ceramide hydrolysis.
Acid ceramidase (encoded by the asah-1 gene) is a lysosomal enzyme that hydrolyzes sphingolipid ceramides into sphingoid bases and free fatty acids at acidic pH. In C. elegans, it contributes to the generation of unique sphingoid bases such as 15-methylhexadecasphinganine and 15-methylhexadecasphing-4-enine, which are structurally distinct from those in other organisms. The protein is localized to lysosomes and can also be secreted, underscoring its role in sphingolipid metabolism and signaling pathways.
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Protein name
Acid ceramidase
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Uniprot ID
O45686
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Gene Name
asah-1
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 17-393 aa (O45686) were fused with 6His tag.
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Protein Species
Caenorhabditis elegans
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Molecular weight
Predictes a molecular mass of 44.80 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 46 kDa band.
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Purity
>87%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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