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Product Name
ARPC1A Polyclonal Antibody
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Description
Polyclonal antibody to ARPC1A
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
ARPC1A antibody; Arc40 antibody; HEL-68 antibody; HEL-S-307 antibody; SOP2Hs antibody; SOP2L antibody; actin-related protein 2/3 complex subunit 1A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 171-350 of human ARPC1A (NP_006400.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - ARPC1A Polyclonal Antibody
Western blot analysis of extracts of U-251MG cells, using ARPC1A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"