ARF6 Polyclonal Antibody

Cat.#: 160203

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Product Information

  • Product Name
    ARF6 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ARF6
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ARF6 antibody; ADP-ribosylation factor 6 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human ARF6 (NP_001654.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - ARF6 Polyclonal Antibody

    Western blot - ARF6 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using ARF6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 10s.

    Immunofluorescence - ARF6 Polyclonal Antibody

    Immunofluorescence - ARF6 Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using ARF6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis (By similarity). Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers (By similarity). Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 (By similarity). Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity).; (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"