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  • Anti-ZNRF2 antibody

Anti-ZNRF2 antibody

Cat.#: 176539

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-ZNRF2 antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to ZNRF2
  • Tested applications
    WB, IHC-P
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    RNF202 antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within human znrf2 aa 181-200.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,500

    IHC-P: 1:50-1:200

  • Validations

    Fig1:; Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ZNRF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig1:; Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ZNRF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Background
  • References
    • Hoxhaj G., et al. 2012. ZNRF2 is released from membranes by growth factors and, together with ZNRF1, regulates the Na+/K+ATPase. J. Cell. Sci. 125:4662-4675.
    • Bian Y., et al. 2014. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics 96:253-262.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"