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Product Name
Anti-WARS / TrpRS antibody
- Documents
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Description
Rabbit polyclonal to WARS / TrpRS
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Tested applications
ELISA, WB, IP
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Species reactivity
Human WARS / TrpRS
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Alternative names
IFI53 antibody; IFP53 antibody; GAMMA-2 antibody; WRS antibody; TrpRS antibody; tryptophanyl-tRNA synthetase antibody; WARS antibody; GAMMA-2 antibody; IFI53 antibody; IFP53 antibody; hWRS antibody; interferon-induced protein 53 antibody; trpRS antibody; tryptophan tRNA ligase 1 antibody; cytoplasmic antibody; tryptophan--tRNA ligase antibody; cytoplasmic antibody; tryptophanyl-tRNA synthetase antibody; Wars antibody; TrpRS antibody; WRS antibody; tryptophan--tRNA ligase antibody; cytoplasmic antibody; tryptophanyl-tRNA synthetase antibody; cytoplasmic antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human WARS / TrpRS (rh WARS / TrpRS; P23381-1; Pro2-Gln471). WARS / TrpRS specific IgG was purified by Human WARS / TrpRS affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human WARS / TrpRS. The detection limit for Human WARS / TrpRS is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
WARS / TrpRS Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
WARS / TrpRS Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
WARS, also known as TrpRS, is an aminoacyl-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. There are two forms of tryptophanyl-tRNA synthetase: a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. They catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. WARS catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon.
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References
- Buwitt U. et al., 1992, EMBO J. 11 (2): 489-96.
- Fleckner J. et al., 1992, Proc Natl Acad Sci. 88 (24): 11520-4.
- Ewalt KL. et al., 2002, Biochemistry. 41 (45): 13344-9.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"