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Product Name
Anti-VWC2 antibody
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Description
Rabbit polyclonal to VWC2
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Tested applications
ELISA, IHC-P
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Species reactivity
Human VWC2
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Alternative names
UNQ739 antibody; PSST739 antibody; G11 antibody; UNQ739 antibody; cradin antibody; PSST739 antibody; A930041G11Rik antibody; MGC131845 antibody; A930041G11Rik antibody; cradin antibody; PSST739 antibody; PSST739 antibody; RP23-305B2.1 antibody; UNQ739 antibody; UNQ739 antibody; UNQ739/PRO1434 antibody; Vwc2 antibody; VWC2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human VWC2 (rh VWC2; NP_055747.1; Met 1-Met 325). VWC2 specific IgG was purified by Human VWC2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human VWC2. The detection limit for Human VWC2 is approximately 0.00975 ng/well.
IHC-P: 0.5-2 μg/mL
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Validations
VWC2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human VWC2 in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein which contains two VWFC domains. VWC2 / Brorin is a BMP antagonist which may play a role in neural development. It promotes cell adhesion. VWC2 / Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2 / Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2 / Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.
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References
- Koike,N. et al., 2007, J Biol Chem. 282 (21):15843-50.
- Elis,S. et al., 2009,Mol Reprod Dev. 76 (11):1043-55.
- Zhang,J.L. et al., 2010,PLoS One. 5 (9):e12846.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"