Anti-Vimentin (4F8) Mouse antibody

Cat.#: 168296

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Product Information

  • Product Name
    Anti-Vimentin (4F8) Mouse antibody
  • Documents
  • Description
    Vimentin (4F8) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IP, IHC-P
  • Species reactivity
    Human
  • Alternative names
    CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin. antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    Antigen: Purified recombinant human Vimentin protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/800

  • Validations

    Western blot detection of Vimentin in Molt-4,K562,COS7,Jurkat,Hela and Vimentin negative cell (Daudi,Ramos,Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.

    Western blot detection of Vimentin in Molt-4,K562,COS7,Jurkat,Hela and Vimentin negative cell (Daudi,Ramos,Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.

    Immunohistochemical analysis of paraffin-embedded human tonsils using Vimentin (4F8) Mouse mAb.

    Immunohistochemical analysis of paraffin-embedded human tonsils using Vimentin (4F8) Mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using Vimentin mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using Vimentin mouse mAb.

    Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-Vimentin mouse mAb (dilution 1:800).

    Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-Vimentin mouse mAb (dilution 1:800).

  • Background
    Swiss-Prot Acc.P08670.Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • References
    • Repression of miR-135b-5p promotes metastasis of early-stage breast cancer by regulating downstream target SDCBPScreening of Recurrence Related MicroRNA in Ductal Carcinoma In Situ and Functional Study of MicroRNA-654-5p.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"