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Product Name
Anti-UGP2 antibody
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Description
Mouse monoclonal antibody to UGP2
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
UDPG antibody; UGP1 antibody; DEE83 antibody; UDPGP antibody; UGPP1 antibody; UGPP2 antibody; EIEE83 antibody; SVUGP2 antibody; UDPGP2 antibody; pHC379 antibody
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Isotype
IgG1
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Preparation
This antigen of this antibody was recombinant protein.
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Clonality
Monoclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500
IHC-P: 1:50-1:100
FC: 1:50-1:100
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Validations
Fig1: Western blot analysis of UGP2 on human liver tissue lysate using anti-UGP2 antibody at 1/200 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-UGP2 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UGP2 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-UGP2 antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of LOVO cells with UGP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
- Background
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References
- Fuhring J I et al. A quaternary mechanism enables the complex biological functions of octameric human UDP-glucose pyrophosphorylase, a key enzyme in cell metabolism. Sci Rep 5:9618-9618 (2015).
- Peng H-L et al. Cloning of a human liver UDP-glucose pyrophosphorylase cDNA by complementation of the bacterial galU mutation. FEBS Lett 329:153-158 (1993).
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