Cart 2

Anti-Ubc9/UBE21 (1D6) Mouse antibody

Cat.#: 168351

Size:

Special Price 441.3 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Anti-Ubc9/UBE21 (1D6) Mouse antibody
  • Documents
  • Description
    Ubc9/UBE21 (1D6) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human, Monkey
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human UBE2I expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    ICC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).

    Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).

    Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.

    Western blot detection of Ubc9 in C6,Jurkat,K562,3T3 and Hela cell lysates using Ubc9 mouse mAb (1:2000 diluted).Predicted band size:18KDa.Observed band size:17KDa.

    Western blot detection of Ubc9 in C6,Jurkat,K562,3T3 and Hela cell lysates using Ubc9 mouse mAb (1:2000 diluted).Predicted band size:18KDa.Observed band size:17KDa.

    Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.

    Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.

    Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining.

  • Background
    Swiss-Prot Acc.P63279.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"