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Product Name
Anti-UBASH3A antibody
- Documents
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Description
Rabbit polyclonal to UBASH3A
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Tested applications
ELISA, IHC-P
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Species reactivity
Human UBASH3A
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Alternative names
ubiquitin associated and SH3 domain containing A antibody; UBASH3A antibody; CLIP4 antibody; STS-2 antibody; TULA antibody; TULA-1 antibody; T-cell ubiquitin ligand 1 antibody; T-cell ubiquitin ligand protein antibody; cbl-interacting protein 4 antibody; suppressor of T-cell receptor signaling 2 antibody; ubiquitin-associated and SH3 domain-containing protein A antibody; ubiquitin associated and SH3 domain containing antibody; A antibody; Ubash3a antibody; RP24-261L18.1 antibody; 5830413C03Rik antibody; C330001M22 antibody; Sts-2 antibody; TULA antibody; TULA-1 antibody; T-cell ubiquitin ligand 1 antibody; suppressor of T-cell receptor signaling 2 antibody; ubiquitin-associated and SH3 domain-containing protein A antibody; TULA antibody; CLIP4 antibody; STS-2 antibody; TULA-1 antibody; TULA antibody; Sts-2 antibody; TULA-1 antibody; C330001M22 antibody; 5830413C03Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human UBASH3A (rh UBASH3A; AAH69511.1; Ala354-Asn623). UBASH3A specific IgG was purified by Human UBASH3A affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human UBASH3A. The detection limit for Human UBASH3A is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
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Validations
UBASH3A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
UBASH3A is a member of the T-cell ubiquitin ligand (TULA) family. This family consists of two members. Both of them can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells, which may occur via its interaction with AIF, an apoptosis-inducing factor. Alternative splicing of UBASH3A gene results in multiple transcript variants. It interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. UBASH3A promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. UBASH3A also exhibits negligigle protein tyrosine phosphatase activity at neutral pH. It may act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. It may also inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.
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References
- Collingwood TS, et al. (2007) T-cell ubiquitin ligand affects cell death through a functional interaction with apoptosis-inducing factor, a key factor of caspase-independent apoptosis.". J. Biol. Chem. 282 (42): 30920-8.
- Smirnova EV, et al. (2008) TULA proteins bind to ABCE-1, a host factor of HIV-1 assembly, and inhibit HIV-1 biogenesis in a UBA-dependent fashion. Virology. 372(1):10-23.
- Wattenhofer M, et al. (2001) Isolation and characterization of the UBASH3A gene on 21q22.3 encoding a potential nuclear protein with a novel combination of domains. Hum Genet. 108(2):140-7.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"