Cart 2

Anti-TREM2 antibody

Cat.#: 175736

Size:

Special Price 241.9 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Anti-TREM2 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to TREM2
  • Tested applications
    WB, IHC-P
  • Species reactivity
    Human, Mouse, Rat, Dog, Pig, Cow, Horse
  • Alternative names
    PLOSL2 antibody; TREM-2 antibody; Trem2a antibody; Trem2b antibody; Trem2c antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human trem2 1-100/230
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
  • Applications

    WB:1:500-2000

    IHC-P:1:400-800

  • Validations

    Fig1: Sample: Raji Cell lysate at 30ug;; Primary: Anti-TREM2 at 1:300 dilution;; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;; Predicted band size: 23 kD; Observed band size: 23 kD

    Fig1: Sample: Raji Cell lysate at 30ug;; Primary: Anti-TREM2 at 1:300 dilution;; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;; Predicted band size: 23 kD; Observed band size: 23 kD

    Fig2: Sample:; MCF-7 Cell (Human) Lysate at 30 ug; Primary: Anti-TREM2 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 23 kD; Observed band size: 23 kD

    Fig2: Sample:; MCF-7 Cell (Human) Lysate at 30 ug; Primary: Anti-TREM2 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 23 kD; Observed band size: 23 kD

    Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig4: Sample:; MCF-7(Human) Cell Lysate at 30 ug; Raw264.7(Mouse) Cell Lysate at 30 ug; Primary: Anti- TREM2 at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 23 kD; Observed band size: 20 kD

    Fig4: Sample:; MCF-7(Human) Cell Lysate at 30 ug; Raw264.7(Mouse) Cell Lysate at 30 ug; Primary: Anti- TREM2 at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 23 kD; Observed band size: 20 kD

    Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

    Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

  • Background
    Q9NZC2

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"