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Product Name
Anti-TNFRSF9 antibody
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Description
Mouse monoclonal antibody to TNFRSF9
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Tested applications
WB, IHC-P, ICC, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ILA antibody; 4-1BB antibody; CD137 antibody; CDw137 antibody
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Isotype
IgG1
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500
ICC: 1:100-1:500
IHC-P: 1:50-1:200
FC: 1:50-1:200
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Validations
Fig1: Western blot analysis of CD137 on HepG2 cell lysate using anti-CD137 antibody at 1/500 dilution.
Fig2: ICC staining CD137 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD137 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining CD137 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-CD137 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD137 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD137 antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of Jurkat cells with CD137 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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References
- Kang SW et al. Anti-CD137 suppresses tumor growth by blocking reverse signaling by CD137 ligand. Cancer Res. pii: canres (2017).
- Hebb JPO et al. Administration of low-dose combination anti-CTLA4, anti-CD137, and anti-OX40 into murine tumor or proximal to the tumor draining lymph node induces systemic tumor regression.Cancer Immunol Immunother.(2017).
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