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Product Name
Anti-Smad5 antibody
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Description
Rabbit polyclonal to Smad5
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse SMAD5
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Alternative names
1110051M15Rik antibody; AI451355 antibody; drosophila antibody; dwarfin-C antibody; DWFC antibody; Dwf-C antibody; JV5-1 antibody; MAD antibody; MAD homolog 5 antibody; Madh5 antibody; MADH5 antibody; mothers against decapentaplegic antibody; mothers against decapentaplegic homolog 5 antibody; mothers against decapentaplegic homolog 5 antibody; mothers against DPP homolog 5 antibody; mothers against DPP homolog 5 antibody; mSmad5 antibody; MusMLP antibody; SMA- and MAD-related protein 5 antibody; SMAD antibody; Smad 5 antibody; SMAD family member 5 antibody; SMAD family member 5 antibody; Smad5 antibody; SMAD5 antibody; MusMLP antibody; Dwf-C antibody; Madh5 antibody; AI451355 antibody; 1110051M15Rik antibody; DWFC antibody; JV5-1 antibody; MADH5 antibody; homolog of antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse SMAD5 (rM SMAD5; P97454; Thr2-Ser465). SMAD5 specific IgG was purified by Mouse SMAD5 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse SMAD5. The detection limit for Mouse SMAD5 is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
Smad5 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse SMAD5 in mouse intestine with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
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References
- Vinayagam A. et al., 2011, Sci Signal. 4 (189): rs8.
- Sangadala S. et al., 2007, J Biomol Struct Dyn. 25 (1): 11-23.
- Riggins GJ. et al., 1996, Nat Genet. 13 (3): 347-9.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"