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Product Name
Anti-Smad3 Rabbit antibody
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Description
Smad3 Rabbit polyclonal antibody
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human Smad3
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IP: 1/20
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Validations
Western blot detection of Smad3 in K562,CHO-K1 cell lysates using Smad3 Rabbit pAb(1:1000 diluted).Predicted band size:48KDa.Observed band size:52KDa.
Western blot detection of Smad3 in Jurkat cell lysates using Smad3 Rabbit pAb(1:1000 diluted).Predicted band size:48KDa.Observed band size:52KDa.
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Background
Swiss-Prot Acc.P84022.Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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