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Product Name
Anti-Smad2 antibody
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Description
Rabbit polyclonal to Smad2
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Tested applications
ELISA, WB, IHC-P, IF, ICC/IF
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Species reactivity
Mouse Smad2
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Alternative names
SMAD antibody; 7120426M23Rik antibody; hMAD-2 antibody; JV18 antibody; JV18-1 antibody; MAD homolog 2 antibody; MAD homolog 2 antibody; Madh2 antibody; MADH2 antibody; MADR2 antibody; Madr2 antibody; mad-related protein 2 antibody; mMad2 antibody; mother against DPP homolog 2 antibody; mothers against decapentaplegic homolog 2 antibody; mothers against decapentaplegic homolog 2 antibody; mothers against DPP homolog 2 antibody; mothers against DPP homolog 2 antibody; Sma- and Mad-related protein 2 antibody; Smad 2 antibody; SMAD family member 2 antibody; SMAD family member 2 antibody; Smad2 antibody; SMAD2 antibody; HSMAD2 antibody; JV18 antibody; MADH2 antibody; MADR2 antibody; JV18-1 antibody; hMAD-2 antibody; Madh2 antibody; Madr2 antibody; mMad2 antibody; Smad-2 antibody; 7120426M23Rik antibody; HSMAD2 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Smad2 (rM Smad2; Q62432; Ser2-Ser467). Smad2 specific IgG was purified by Mouse Smad2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Mouse Smad2.
IHC-P: 0.1-3 μg/mL
ICC/IF: 0.5-1.5 μg/mL
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Background
SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.
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References
- Feng. et al., 2002, Mol Cell. 9 (1): 133-43.
- Zhu Y. et al., 1997, J Biol Chem. 272 (15): 10035-40.
- Zi Z. et al., 2012, FEBS Lett. 586 (14): 1921-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"