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Anti-SLC9A1 antibody

Cat.#: 175699

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-SLC9A1 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to SLC9A1
  • Tested applications
    WB, IHC-P
  • Species reactivity
    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
  • Alternative names
    APNH antibody; NHE1 antibody; LIKNS antibody; NHE-1 antibody; PPP1R143 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human nhe1 470-550/815
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    IHC-P:1:400-800

  • Validations

    Fig1: Sample:; Brain(Rat) lysate at 30ug;; Liver(Rat) lysate at 30ug;; Primary: Anti-NHE1 at 1:200 dilution;; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;; Predicted band size : 91kD; Observed band size : 91kD; We are unsure as to the identity of these extra bands

    Fig1: Sample:; Brain(Rat) lysate at 30ug;; Liver(Rat) lysate at 30ug;; Primary: Anti-NHE1 at 1:200 dilution;; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;; Predicted band size : 91kD; Observed band size : 91kD; We are unsure as to the identity of these extra bands

    Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig3: Sample:; molt-4(human)cell Lysate at 40 ug; Primary: Anti- Sodium hydrogen exchanger 1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 91kD; Observed band size: 91 kD

    Fig3: Sample:; molt-4(human)cell Lysate at 40 ug; Primary: Anti- Sodium hydrogen exchanger 1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 91kD; Observed band size: 91 kD

  • Background
    P19634
  • References
    • Proc. Natl et al. A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange. Proc. Natl. Acad. Sci. U.S.A. 93:12631-12636(1996)
    • Pang T. et al. Role of calcineurin B homologous protein in pH regulation by the Na+/H+ exchanger 1: tightly bound Ca2+ ions as important structural elements. Biochemistry 43:3628-3636(2004)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"