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Product Name
Anti-SETD7/SET7/9 antibody
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Description
Mouse monoclonal to SETD7/SET7/9
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Tested applications
WB
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Species reactivity
Human SETD7 / SET7 / 9
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Alternative names
1600028F23Rik antibody; FLJ21193 antibody; H3K4MT antibody; KIAA1717 antibody; KMT7 antibody; KMT7 antibody; mKIAA1717 antibody; SET7 antibody; Set7 antibody; Set7/9 antibody; SET7/9 antibody; SET9 antibody; Setd7 antibody; SETD7 antibody; KMT7 antibody; SET7 antibody; SET9 antibody; SET7/9 antibody; KMT7 antibody; Set7 antibody; H3K4MT antibody; Set7/9 antibody; mKIAA1717 antibody; 1600028F23Rik antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SETD7 / SET7 / 9 (rh SETD7 / SET7 / 9; NP_085151.1; Asp2-Lys366). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% treha
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20μg/ml
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Validations
SETD7 / SET7 / 9 Antibody, Mouse MAb, Western blot
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Background
Histone-lysine N-methyltransferase SETD7, also known as SET domain containing (lysine methyltransferase) 7, SET7/9, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, and SETD7, is a member of the histone-lysine methyltransferase family and SET7 subfamily. SETD7 is widely expressed and expressed in pancreatic islets. SETD7 contains three MORN repeats and one SET domain. SETD7 plays a central role in the transcriptional activation of genes such as collagenase or insulin. As a protein lysine methyltransferase (PKMT), SETD7 also has methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding in substrate proteins. The mono-methyltransferase activity of SETD7 is achieved by disrupting the formation at near-attack conformations for the dimethylation reaction. SETD7 is also a novel coactivator of NF-kappaB and plays a role in inflammation and diabetes.
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References
- Wang, H. et al., 2002, Mol Cell 8 (6): 1207-17.
- Jacobs, SA. et al., 2002, Nat. Struct. Biol. 9 (11): 833-8.
- Xiao B, et al., 2003, Nature. 421 (6923): 652-6.
- Martens, JH. et al., 2003, Mol. Cell. Biol. 23: 1808-1816.
- Couture, JF. et al., 2006, Nat Struct Mol Biol 13 (2): 140-6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"