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Product Name
Anti-SERCA2 ATPase Rabbit antibody
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Description
SERCA2 ATPase Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
DD; DAR; ATP2B; SERCA2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human SERCA2 ATPase
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000-1/10000
IHC: 1/20
ICC/IF: 1/50
FC: 1/20-1/100
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Validations
Immunohistochemistry of SERCA2 ATPase in paraffin-embedded Human colon cancer tissue using SERCA2 ATPase Rabbit pAb at dilution 1/50
Western blot detection of SERCA2 ATPase in K562,Hela cell lysates using SERCA2 ATPase Rabbit pAb(1:1000 diluted).Predicted band size:115kDa.Observed band size:115kDa.
Immunocytochemistry of SERCA2 ATPase(green) in Hela cells using SERCA2 ATPase Rabbit pAb at dilution 1/50, and DAPI(blue)
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Background
Swiss-Prot Acc.P16615.This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle (PubMed:16402920). Acts as a regulator of TNFSF11-mediated Ca2+ signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"